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Study on cytotoxicity of slightly acidic electrolyzed water to oral fibroblasts

2025-03-20 16:13:26

Slightly Acidic Electrolyzed Water (SAEW) is water produced by electrolysis of dilute hydrochloric acid or sodium chloride solution. Its main bactericidal component is hypochlorous acid (HClO), with a pH range of 5.0 to 6.5 and an effective chlorine content of 10 to 80 mg/L. This water is colorless, transparent, has a slight chlorine odor, is low in corrosiveness and is environmentally friendly. It is a safe and efficient disinfectant.

 

Biological characteristics and culture conditions of oral fibroblasts

Oral fibroblasts are a type of adherent cell derived from oral mucosal tissue with a fibroblast-like morphology and are widely used in cell and molecular research.

 

SAEW achieves efficient killing of biological cells through multiple mechanisms such as destroying cell membranes, inducing oxidative stress, interfering with metabolic pathways, denaturing proteins, and changing cell morphology.

 

The existing literature on the interaction between slightly acidic electrolyzed water (SAEW) and oral fibroblasts mainly focuses on the effect of SAEW on the disinfection of the water channel of the comprehensive oral treatment table and its cytotoxicity experiment. The specific research is as follows:

 

Study on the disinfection of water channels of dental comprehensive treatment tables by SAEW

The literature mentions that SAEW, as a new type of disinfectant, generates an acidic aqueous solution with a pH value of 5.0 to 6.5 by electrolyzing softened water. Its main bactericidal component is hypochlorous acid (HClO). Studies have shown that SAEW has a significant disinfection effect on dental unit waterways (DUWLs) and has a sustained antibacterial effect. The experimental results show that different concentrations of SAEW (40 mg/L, 20 mg/L, 10 mg/L) can achieve a disinfection pass rate of more than 99.92% within 1 minute, and have low toxicity to fibroblasts (TR146 cells), with a cell survival rate of more than 98%.

 

Sterilization mechanism and application prospects of SAEW

The sterilization mechanism of SAEW is mainly through the damage of bacterial cell membrane by hypochlorous acid, which leads to the death of microorganisms. Research also shows that SAEW has broad application prospects in the medical and health field, including air disinfection, surface disinfection of hospital environment objects, oral medical treatment, and disinfection of medical staff's hands and skin.

 

Application of SAEW in oral medicine

As a non-toxic and non-irritating disinfectant, SAEW has been used to disinfect the waterways of comprehensive oral treatment tables. It can effectively reduce the infection rate in the oral department and protect the safety of patients and medical staff.

 

The existing literature on the interaction between SAEW and oral fibroblasts mainly focuses on its disinfection effect and toxic effects on cells, indicating that SAEW is a safe and effective disinfectant suitable for disinfection needs in oral medical environments.

 

The specific mechanism of action of SAEW on oral fibroblasts may involve the following aspects:

At low concentrations, SAEW had no significant toxicity to fibroblasts and may indirectly protect fibroblasts by maintaining the stability of the oral microenvironment and reducing the risk of infection.

At high concentrations, SAEW may lead to impaired fibroblast function by inducing oxidative stress and affecting the TGF-β/Smad2 signaling pathway.

The antibacterial and antibiofilm effects of SAEW may indirectly reduce the inflammatory response around fibroblasts, thereby promoting tissue repair.

Future studies need to further explore the specific molecular mechanisms of SAEW on fibroblasts, especially its effects under different concentrations and environmental conditions.

 

What are the long-term effects of SAEW on oral fibroblasts ?

Cytotoxic effect: Several studies have shown that SAEW has a certain degree of cytotoxicity to oral fibroblasts at a certain concentration. For example, when the concentration of SAEW reached 1 mg/mL, it had a significant effect on the viability of TR146 cells, but this toxicity could be neutralized by BSA pretreatment. In addition, the effect of SAEW on cell proliferation was more obvious at high concentrations (e.g., above 5 mg/mL), but this effect could be neutralized by BSA.

 

Disinfection effect and safety: SAEW has a significant disinfection effect in the oral environment and can effectively kill a variety of bacteria in the oral cavity, including Candida albicans and Streptococcus mutans. However, its disinfection effect may be affected by organic matter in the oral cavity (such as protein, vitamin C, etc.).

 

Effects of long-term use: Although SAEW has low toxicity to oral fibroblasts in the short term, long-term use still requires caution. Studies have shown that the disinfection effect of SAEW may weaken over time, and high concentrations of SAEW may cause irreversible damage to cells.

 

Limitations of clinical application: Although SAEW has potential application value in oral care, its safety and effectiveness in long-term use still need further study. Current research mostly focuses on short-term experiments and disinfection effects, and lacks a systematic evaluation of long-term effects.

 

In summary, the long-term effects of SAEW on oral fibroblasts are mainly manifested as certain cytotoxic effects, but their toxicity can be reduced by appropriate pretreatment (such as BSA neutralization). In addition, the application of SAEW in oral disinfection has a significant effect, but its safety and effectiveness in long-term use still need further research and verification.

 

Chlory has been committed to developing efficient and safe disinfection and care products. Its hypochlorous acid products are well-known for their unique slightly acidic electrolyzed water formula. According to Chlory, the product generates hypochlorous acid through electrolysis technology, which can effectively kill harmful bacteria in the mouth while maintaining a slightly acidic state and is less irritating to the oral mucosa and fibroblasts.

 

As people pay more and more attention to oral health, slightly acidic electrolyzed water is gradually gaining favor in the market as a new oral care ingredient. Chlory is expected to occupy an important position in this field with its advanced technology and scientific product design.

 

In the future, Chlory plans to further expand its product line and strengthen cooperation with scientific research institutions to promote the development and application of more oral care products based on slightly acidic electrolyzed water.


For more information about Chlory and hypochlorous acid generators, visit www.chlory.com or contact info@chlory.com