The slightly acidic electrolyzed water containing 10 mgL-30 mgL of initial available chlorine was used for 5 min, and the average killing logarithm of Escherichia coli in the suspension was 2.0-2.48. The slightly acidic electrolyzed water was atomized by a sterilization atomizer and acted for 120 min, and the average sterilization rate of natural bacteria in the air of the confined space could reach more than 90%. Conclusion Slightly acidic electrolyzed water has a certain bactericidal ability, and it has a good sterilization effect on natural bacteria in the air of a confined environment after atomization.
As a mild and safe disinfectant, hypochlorous acid is widely used in disinfection of various environments, including laboratories. Hypochlorous acid can effectively disinfect the air and surfaces of objects through spraying, and it is non-irritating to the human body and leaves no residue, making it suitable for use in laboratories and other environments. In addition, the hypochlorous acid spray system can atomize the disinfectant into tiny particles that can float in the air for a long time, thereby achieving a comprehensive disinfection effect.
Hypochlorous acid spray is not only suitable for laboratory environments, but also used in many other occasions, such as hospitals, nursing homes, schools, etc., for space sterilization and deodorization, formaldehyde removal, and mite removal. Therefore, the application of hypochlorous acid spray in the laboratory is reasonable and effective.
Specific application cases of hypochlorous acid spray disinfection in laboratories include the following aspects:
- In one study, 8,700 ppm hypochlorous acid solution was used for dry mist disinfection and the results showed that this concentration of hypochlorous acid solution was effective in killing SARS-CoV-2 and influenza A viruses. Experiments have shown that as the disinfectant concentration increases, the infectivity of the virus significantly decreases, indicating that hypochlorous acid spray disinfection has a good inactivation effect on these viruses in a laboratory environment.
Figure 1. Inactivation of viruses by the dry fogging of disinfectants. Changes in the viral infectious titers (TCID50 values) of SARS-CoV-2 (A and C) and influenza A virus (B and D) upon dry fogging with hypochlorous acid solution (HAS) (A and B), hydrogen peroxide solution (H2O2) (C and D), or distilled water (DW) (A, B, C, and D) were evaluated, as described in the Materials and methods. Horizontal dotted lines in the graphs show the detection limit of the viral titer. *P < 0.0001 compared with DW using a two-way ANOVA. n.s., not significant. Each data point represents the average and standard deviation obtained from more than three repeated experiments.
Another study demonstrated significant microbial load reduction following the use of sodium hypochlorite biocide in a PPE sterilization chamber. The findings highlight the importance of immediate disinfection of PPE, especially during the SARS-CoV-2 pandemic. Sodium hypochlorite shows high efficacy against a variety of bacteria and fungi, including Gram-positive and Gram-negative bacteria and a variety of fungal strains.
The disinfection potential of hypochlorous acid (HOCl) in suspension has been studied for air cleaning applications. By aerosolizing HOCl solution, studies have found that it has efficient disinfection capabilities in the air and can control indoor microbial contamination within legal limits. These preliminary results suggest that hypochlorous acid should be further evaluated as an air purification method to complement existing infection control concepts.
In hospital settings, sodium hypochlorite is used to disinfect various environmental surfaces (e.g. floors, walls, equipment, etc.). Studies have shown that sodium hypochlorite has significant killing effects on multiple drug-resistant bacteria (such as C. difficile, S. aureus, MRSA), fungi (such as Candida genus) and fat-soluble viruses. Its efficiency is affected by contact time and concentration, but overall it shows a high disinfection effect.
The application of hypochlorous acid disinfectants in pet cleaning and care has also been verified. Experiments have shown that hypochlorous acid disinfectant can effectively inhibit the cytopathic effects of various viruses on cells, and can completely lose their infectivity in 30 minutes at room temperature. Specifically, hypochlorous acid disinfectant has a significant killing effect on canine distemper virus, canine parvovirus BJ-81 strain, feline distemper virus BJ-08 strain and feline calicivirus BJ-102 strain.
Compared with other disinfectants (such as alcohol and hydrogen peroxide), the effectiveness of hypochlorous acid spray disinfection has the following characteristics:
Bactericidal effect: Hypochlorous acid (HOCl) has higher killing activity against bacteria at the same concentration and is more effective than sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2). For example, in spray form, hypochlorous acid is highly effective against E. coli and salmonella, and 20 minutes of exposure at a concentration of 0.01% is enough to reduce Staphylococcus epidermidis by 99.5%.
Safety: Due to its non-toxic properties, hypochlorous acid is harmless to operators and does not require the wearing of personal protective equipment, which provides additional advantages for disinfection applications. In addition, hypochlorous acid disinfectants have low concentration, low odor, low irritation, high safety, and wide application range.
Stability : Sodium hypochlorite is a strong oxidant, its oxidizing ability is 25 times that of chlorine, and its disinfecting ability is second only to ozone and higher than chlorine.
Applications: Hypochlorous acid has been proposed as an effective disinfectant in a variety of scenarios, ranging from food production, handling and preparation to medical applications including chronic wound care and eyelid infection management. Its application in the food industry is of interest, particularly in the control of biofilm formation on bacterial outer surfaces.
Studies have shown that at low concentrations, hypochlorous acid has less impact on human health and is even considered a safe and effective disinfectant . Low-concentration hypochlorous acid nasal irrigation solution has a significant inhibitory effect on bacteria, fungi and viruses, and also has a certain effect on rhinovirus infection of nasal mucosal epithelial cells.
In general, hypochlorous acid spray disinfection can be used as an effective disinfection method when used appropriately and the concentration controlled.
The test results of laboratory use of hypochlorous acid spray disinfection are as follows:
Figure 2. The killing effect of SAEW on Bacillus subtilis black variant spore
Exploring safe, effective and rapid disinfection measures has always been a research hotspot in the field of disinfection, and is also the driving force behind BLUEWAV's continuous progress.
Currently internationally recognized standards for ideal disinfectants include: broad bactericidal spectrum, rapid action; stable performance, easy to store and transport; non-toxic, odorless, non-irritating, non-teratogenic, carcinogenic, or mutagenic; easily soluble in water, No coloring, easy to remove, no environmental pollution, low concentration, easy to use, etc. With years of research and practice, BLUEWAV hypochlorous acid water has been gradually exported to countries around the world for its broad sterilization spectrum, rapidity, easy use of equipment, low cost, and long service life . Compared with acidic oxidation potential water, it does not produce by-products such as ozone . The pH value is close to 7 and can be adjusted from 3 to 7. It is almost non-corrosive to objects. It uses dilute hydrochloric acid as raw material and has low cost. The generated products will change with the air. Evaporation is not easy to remain in the environment and is safer and more environmentally friendly . It has been gradually used in food, agriculture, medical and other fields . Hypochlorous acid water has a significant sterilizing effect on natural bacteria and Escherichia coli on the surface of cucumbers . The average killing log values after 30 disinfection tests reached 2.18 and 3.08 respectively . It is an ideal food disinfectant.
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